Title : Autologous fat grafting preserving adipose derived cells, new technologies and their impact on skin and hair care
Besides the fact that fat grafting gained popularity, isolation of ADCs (Adipose Derived Cells) and fat tissue manipulation still remains controversial. In 2001, a putative Stem cell population was isolated within the adipose stromal compartment. Since then, many studies exhibited and confirmed the abundance of adult mesenchymal cells, endothelial progenitor cells and growth factor-producing cells derived from fat tissue. Isolation of those cells, its activation and their immediate use for fat transplant still remain a challenge. The purpose of this study is to show our evolution from chemical to mechanical dissociation of those cells from the fat tissue stroma since 2002 Methods / Technique: Adipose tissue is collected from the abdomen of patients undergoing liposuction. The fat is harvested and processed using two selective methods. Chemical: 100 mls of Fat is submitted to the collagenase isolation method. The stromal vascular fraction is centrifuge and the infranated pellet is reserved . Mechanical: After 100 mls of Fresh Adipose Tissue was washed with a saline solution, a collagenase free Mechanical shear Force maneuver is made, generating a gradient force that detaches the ADCs from the adipose tissue stroma. The presence of mesenchymal stem cells isolated in the pellet was confirmed by Indirect Immunofluorescence and Flow Cytometer analysis in a selective sample data in both methods Results / Complications: From February 2002 to October 2020, 741 patients benefited from autologous fat transplant preserving ADCs. Chemical Dissociation was just used to compare the final pellet counting . Mechanical Dissociation. Was the selected method for human use . The donor site was the abdomen. An average of 20 to 30 million mesenchymal stem cells / 100ml of processed lipoaspirate was isolated with the Mechanical method compared to 30 to 40 million from the Chemical Method . Conclusion: Up to now, adipose-derived cells isolation and fat tissue manipulation was mainly be done in the lab or using expensive processing machines and collagenase. The mechanical method has shown to be reproducible is collagenase free and has been used since 2006 in a long-term evaluation.