Title : Human amniotic epithelial cell derived extracellular vesicles promote hair growth by activating wnt/?-catenin and shh signaling
Abstract:
Background: Hair loss is a common condition, but there are significant limitations to present treatments. The promotion in the hair follicle cycle from the telogen to the anagen stage is the significant mechanism to regulate hair regrowth. Human amniotic epithelial cells (HAECs)-derived extracellular vesicles (EVs) have been considered an attractive treatment for hair growth.
Methods: hAECs from human amniotic membranes were isolated and identified with specific markers. hAEC-EVs were isolated from the hAEC culture medium and identified with a nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and western blot. The depilated C57BL/6 mice were treated with hAECs or hAEC-EVs. H&E staining, RT-PCR and immunofluorescence staining were used to analyze the promotion of hairgrowth. And Ki67 immunofluorescence staining and wound healing assays were used to evaluate the effect of hAEC-EVs on the proliferation and migration abilities of human hair follicle cells.
Results: The hAEC and hAEC-EVs promoted the regrowth of back hair of mice after depilation. The hAEC-EVs profoundly accumulated proliferation and migration of hair follicle cells. The therapeutic effects of hAEC-EVs may be due to upregulation of hair growth-promoting signaling pathway including Wnt/β-catenin and Shh signaling.
Conclusions: hAEC-EVs promote the hair growth via activation of Wnt/β-catenin and Shh signaling pathway. And hAEC-EVs could be developed as a potential therapeutic approach for hair loss.
Keywords: Human Amniotic Epithelial Cell, Extracellular Vesicles,hair growth
