Title : A comparative study of in-vitro efficacy of ozenoxacin vs mupirocin vs fusidic acid against gram positive clinical isolates
Abstract:
Primary objective is to compare in vitro efficacy of ozenoxacin vs mupirocin vs fusidic acid in terms of MIC against gram positive infections.
Materials and Methods: It is a cross-sectional observational study. Patients more than 2 years of age with pyoderma (which includes folliculitis, furunculosis, carbuncle, impetigo, sycosis barbae) were included in the study. Pregnant and lactating women were excluded from study. The procedure includes inoculating collected pus in blood and Mcconkey agar plates with a backup broth. The identification and sensitivity pattern were performed by Vitek 2, Biomerieux. MIC was determined by the broth microdilution method as recommended by the Clinical and Laboratory Standards Institute (CLSI). Susceptibility was determined using breakpoints set by the CLSI for ozenoxacin and that of EUCAST breakpoints will be used for fusidic acid and mupirocin against staphylococci. MIC50 and MIC90 values were calculated for each antimicrobial agent.
Result and Discussion: A total of 70 isolates were collected from outpatients of dermatology department. Out of 70 samples inoculated 63 were gram positive, with 4 no growth and three gram negative isolates. Gram positive isolates were identified as S. aureus (n = 37), MRSA (n = 7), S. epidermidis (n= 10), S. hemolyticus (n=4), S. hominis (n=3), S. capitis (n=1), S. saprophyticus(n=1). Fusidic acid was more potent against S.aureus isolates (n = 37), with a MIC90 of 0.55 mg/l as compared to mupirocin (MIC90- 8.5 mg/dl) and ozenoxacin (MIC90- 1mg/dl). MIC50 and MIC90 of mupirocin, fusidic acid and ozenoxacin for MRSA were 1.0625,0.125,0.25 and 22.4,17.6 and 32mg/dl respectively.
Conclusion: We found Ozenoxacin to be having the lowest MIC50 than other two antibiotics against Staph. Aureus. 6.3% cases of isolates were resistant to mupirocin. Methicillin resistant staph aureus had higher MIC90 for all the antibiotics.
Audience Take Away Notes:
The audience will get insight about the effectiveness of topical antibiotic and it will eventually be helpful for choosing the best option while prescribing topical antibiotic for any skin infections. Further studies can be done on a larger scale to increase sample size and can make more informed decisions. Will improve treatment quality of the patients
