Title : Oxyresveratrol attenuates inflammation in human keratinocyte via regulating NF-KB signaling and ameliorates eczematous lesion in DNCB-induced dermatitis mice
Abstract:
Background: Oxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is Artocarpus lakoocha, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not been clearly demonstrated.
Objectives: To investigate the anti-inflammatory effects of ORV on keratinocytes and determine the treating efficacy of ORV cream on dermatitis mouse model.
Material and Methods: The effect of ORV was examined on human immortalized and primary skin cells exposed to bacterial components including peptidoglycan (PGN) and lipopolysaccharide (LPS) and 2,4-Dinitrochlorobenzene (DNCB)-induced dermatitis mouse model. PGN and LPS were used to induce inflammation on immortalized keratinocytes (HaCaT) and human epidermal keratinocytes (HEKa). We then performed MTT assay, Annexin V and PI assay, cell cycle analysis, real-time PCR, ELISA and Western blot in these in vitro models. Clinical manifestation score, H&E staining, immunohistochemistry (IHC) staining with CD3, CD4 and CD8 markers were used to evaluate the effects of ORV in in vivo model of skin inflammation using BALB/c mice.
Results: Pretreatment of HaCaT and HEKa cells with ORV inhibited pro-inflammatory cytokine production through inhibition of NF-kB pathway. In DNCB-induced dermatitis mouse model, ORV treatment reduced lesion severity, and skin thickness and numbers of CD3, CD4 and CD8 T cells in the sensitized skin of mice.
Conclusion: ORV treatment can ameliorate inflammation in the in vitro models of skin inflammation and in vivo models of dermatitis, suggesting a therapeutic potential of ORV for treatment of skin diseases particularly eczema.
Audience Take Away Notes:
- More than its antioxidative effect, oxyresveratrol also possesses anti-proliferative and anti-inflammatory effects. Therefore, it can be a potential treatment for dermatitis
- With the determined characteristics of oxyresveratrol, we believed that products containing oxyresveratrol could contribute variety to the treatment and cosmetic markets
- Further studies on oxyresveratrol should be conducted on the JAK/STAT pathway, the antibacterial effect, and other disease models in dermatology
