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Title: Strategies to overcome ischemia in wound healing

Farzad Hayati

University of Tehran, Iran

Biography

I am veterinary surgeon (DVM: Ferdowsi University of Mashhad, DVSc: University of Tehran) from Iran. Since 2009, I focused my research on skin wound healing. I have clinical experience about animal wound treatment. My main concern is ischemia during wound healing and I registered a patent about making ATP-liposomes to deliver energy to cells in ischemic wounds.  I think one of the main obstacles in treatment of challenging wounds like diabetic and burn wounds is ischemia. I am eager to communicate with experts in dermatology and finding new ideas about wound treatment and hope to find new solutions.

Abstract

Burns are among the most devastating of all injuries, with outcomes spanning the spectrum from physical impairments and disabilities to emotional and mental consequences. Obviously any consideration of burn wound healing must bear in mind the general principles of the wound healing process. But biological process acting to repair burn wounds has some differences from those acting to repair any other wounds. One of the most obvious differences between burn and disruptive or incisional wounds is in the effect of the injury upon blood vessels at the site of injury. In 1953 Jackson described three zones in burn wound based on the severity of destruction and blood flow alterations: zone of coagulation, zone of ischemia and zone of hyperemia. The reversible nature of tissue damage in zone of ischemia at the very early stage of the burns suggested that this zone could be salvaged when tissue necrosis associated with progressive ischemic insult was prevented. Decrease in blood and oxygen delivery to the wound cells results in significantly decreased cellular energy supply, which impacts nearly every aspect of the healing process. Reduced blood flow or disturbed oxygen supply results in the discrepancy of energy production and utilization. Depletion of high-energy phosphates is the fundamental cause of tissue damage. Healthy cells must maintain a high content of ATP, and almost all energy-requiring processes in cells are driven, either directly or indirectly, by hydrolysis of ATP. Previous studies show only a small amount of ATP can be taken up by the cells, most likely through the membrane pores. Furthermore, the half-life of free ATP in blood circulation is less than 40 s, limiting its efficacy as a bioenergetic substrate. We have developed and used unilamellar lipid vesicles that contain magnesium-ATP for intracellular ATP delivery, and preliminary results indicate that this new energy delivery technique can provide a significant protective effect to ischemic tissues of zone of ischemia in burn wounds. This article reports how cells lose their energy and the previous attempts to save the zone of ischemia and our encapsulation process and results with ATP delivery technique.